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Time- and Wavelength-Resolved Fluorescence Detection for Capillary Zone Electrophoresis with Axial Excitation

✍ Scribed by Angela Fultz; Todd M. Branch; Vahid Majidi

Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
210 KB
Volume
57
Category
Article
ISSN
0026-265X

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✦ Synopsis

Capillary zone electrophoresis (CZE) has become a powerful tool in separation science. Detection methods for this technique, however, have not yet matured. We describe here two axial optical detection methods with time and wavelength resolution for CZE. CZE separation of rhodamine dyes was carried out in a 200-mm capillary. A 100-mm fiberoptic cable was inserted into the capillary to the edge of the observation window. The fiberoptic was used to transmit laser radiation, which served as the excitation source for fluorescence spectroscopy. Separation of rhodamine 590 from rhodamine 610 and kiton red was achieved. Time-resolved detection allowed the analysis of protein-rhodamine B isothiocyanate conjugates as well as detection of the sensitized fluorescence of fluorescein in the presence of coumarin.

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