TIME- AND CONCENTRATION-DEPENDENCE OF THE GROWTH-PROMOTING ACTIVITY OF INSULIN AND HISTAMINE IN TETRAHYMENA. APPLICATION OF THE MTT-METHOD FOR THE DETERMINATION OF CELL PROLIFERATION IN A PROTOZOAN MODEL
✍ Scribed by HARGITA HEGYESI; GYÖRGY CSABA
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 129 KB
- Volume
- 21
- Category
- Article
- ISSN
- 1065-6995
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✦ Synopsis
The unicellular ciliate Tetrahymena pyriformis was treated with different concentrations of insulin or histamine and at different time points the cell density was measured, using a tetrazolium-based semiautomated colorimetric assay (MTT). The assay was suitable to determine the rate of cell proliferation of Tetrahymena. Insulin in each concentration significantly elevated the cell count up to 3 h. After that, it was neutral or its effect was insignificant. Histamine at 10 5 concentration diminished cell count at 3, 5, 7 and 24 h. At 10 6 concentration there was no difference and at 10 7 concentration it enhanced cell division up to 5 h, after that there being no difference. The two hormones have cell division promoting activity for cells of higher animals and the experiments demonstrate this effect already at a unicellular level.