Tightly-bound non-histone proteins in different nucleosome-like subpopulations from pig kidney chromatin
✍ Scribed by Dr. Paola Caiafa; Antonella Tomassetti; Stefania Mastrantonio; Anna Reale; Maurizio Spinelli; Roberto Strom
- Book ID
- 102789266
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 542 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0263-6484
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✦ Synopsis
By differential sucrose gradient centrifugation of pig kidney chromatin in the presence or absence of Na-EDTA and under varying ionic strength conditions, three nucleosome-like subpopulations with different buoyant densities can be obtained. These particles, on the basis of their histones and HMG protein pattern, of the 5-methylcytosine level of their DNA and of the RNA polymerase activity associated with them, can be considered as originating from chromatin fractions differently involved in gene expression.
Two-dimensional electrophoresis of the tightly-bound non-histone proteins shows a distinct pattern for each subpopulation, such protein components being notably present in restricted numbers but in high amounts in the subpopulation which was apparently derived from condensed heterochromatin.