CONTENTS......Page 4
Preface......Page 12
1. Evolution of the Transporting Epithelium Phenotype......Page 13
The Development of Tight Junctions......Page 16
The Development of Polarity: The Role of the β-Subunit of the Na[sup(+)],K[sup(+)]-ATPase......Page 17
Na[sup(+)],K[sup(+)]-ATPase, Cell Attachment and the Position of the TJ......Page 18
Evolution of the Na[sup(+)],K[sup(+)]-ATPase......Page 20
Evolution of Junction Proteins......Page 21
The Dawn of Metazoans and Transporting Epithelia......Page 23
Na[sup(+)],K[sup(+)]-ATPase and Cell Adhesion: Role of the β-Subunit in Cell Attachment......Page 24
Identification of Occludin from Different Species......Page 31
Localization of Occludin at TJs......Page 32
Expression of Occludin in Vitro......Page 33
Functional Analysis of Occludin Domains......Page 34
Occludin Interacting Proteins......Page 36
Roles of Occludin Phosphorylation......Page 38
Regulation of Occludin by Signaling Molecules......Page 39
Occludin in Cancer Cells......Page 40
Channel-Like Properties of Tight Junctions......Page 45
The Claudin Family and Its Extended Relatives......Page 46
Lessons from Cell Culture Models......Page 49
Lessons from Heritable Human Diseases......Page 50
Lessons from Mutant Mice......Page 51
4. JAM Family Proteins: Tight Junction Proteins That Belong to the Immunoglobulin Superfamily......Page 55
General Features of JAM Family Proteins......Page 56
Functions of JAM Family Proteins......Page 60
Identification of Cingulin as a Tight Junction-Associated Protein......Page 66
Molecular Structure and Evolution of Cingulin......Page 67
The Head Domain of Cingulin Is Essential for Its Junctional Recruitment......Page 69
Cingulin Is Maternally Expressed and Is an Early Component of Cell-Cell Junctions in Developing Vertebrate Embryos......Page 70
Putative Functions and Regulation of Cingulin......Page 72
Paracingulin......Page 73
6. ZO Proteins and Tight Junction Assembly......Page 76
Role as Molecular Scaffold of Tight Junction Proteins......Page 77
ZO Proteins as Cytoskeletal Linkers......Page 79
Organization of Signal Transduction Pathways and Transcription Factor Complexes That Regulate Junction Structure and Function......Page 80
Extension of the Paradigm: ZO Proteins and Global Organization of Cell-Cell Junctions......Page 82
TJ Proteins Participate in Maintaining the Equilibrium between Proliferation and Differentiation......Page 88
Conditions Upon Which TJ Proteins Are Found at the Nucleus......Page 89
Signals Contained in the Sequence of TJ Proteins That Facilitate Membrane-Nuclear Shuttling......Page 92
A Protein Involved in Chromatin Remodeling Colocalizes with ZO-1 at the TJ......Page 98
Nuclear Matrix and TJ Proteins......Page 101
Interaction of TJ Cortical Proteins with Molecules Involved in RNA Processing and Transcriptosome Assembly......Page 104
TJ Proteins Associate with Transcription Factors at the Nucleus and the Plasma Membrane......Page 105
8. Tight Junctions and the Regulation of Epithelial Cell Proliferation and Gene Expression......Page 113
Junctional Plaque Proteins......Page 114
ZO-1 and ZONAB in the Regulation of Cell Proliferation......Page 117
ZONAB and the Regulation of Gene Expression......Page 119
Tight Junctions and the Ras Pathway in the Regulation of Cell Proliferation......Page 120
9. Tight Junction Proteins and Cancer......Page 128
TJ Proteins......Page 129
TJ Proteins and Cancer......Page 130
The TJ and Viral Oncoproteins......Page 136
10. Regulation of Paracellular Transport across Tight Junctions by the Actin Cytoskeleton......Page 147
Modulation of Myosin Light Chain-Phosphorylation in the Perijunctional F-Actin Ring Influences TJ Function......Page 148
Modulation of Barrier Function by Rho GTPases......Page 149
Modulation of Barrier Function by Cytokines......Page 151
11. Regulation of Tight Junctions' Functional Integrity: Role of a Urinary Factor, Lipids and Ouabain......Page 158
Regulatory Factors of the Tight Junctions......Page 159
Urinary Factors......Page 161
Lipids......Page 164
Ouabain......Page 167
12. Tight Junctions during Development......Page 176
Epithelial Differentiation in the Mouse Embryo......Page 177
Three Phases of Tight Junction Biogenesis......Page 178
Cell Adhesion and Regulation of Tight Junction Assembly......Page 181
Tight Junctions in Other Mammalian Preimplantation Embryos......Page 182
13. Tight Junctions and the Blood-Brain Barrier......Page 187
Structure of Blood-Brain Barrier Tight Junctions......Page 188
Molecular Composition of Blood-Brain Barrier Tight Junctions......Page 190
Regulatory Mechanisms in the Blood-Brain Barrier......Page 196
The Radial Component of CNS Myelin Are Tight Junctions......Page 208
OSP/Claudin-11 Is Essential for the Formation of CNS Myelin TJs......Page 209
Myelin Compaction Is Dependent on Both OSP/Claudin-11and PLP/DM20......Page 211
Regulation of Migration and Proliferation; OSP/Claudin-11 and Associated Proteins......Page 213
15. Tight Junction Modulation and Its Relationship to Drug Delivery......Page 218
Structural Characteristics of Tight Junctions......Page 219
Enhancing Drug Delivery via Modulation of the TJs......Page 221
Discovery of Zonula Occludens Toxin......Page 222
Identification of the Zot Receptors......Page 223
Zot as a TJ Modulator for Improved Drug Delivery......Page 224
E......Page 232
M......Page 233
P......Page 234
Z......Page 235