Thyroid Hormone Nuclear Receptor

✍ Scribed by Michelina Plateroti, Jacques Samarut

• Publisher: Springer New York;Humana Press
• Year: 2018
• Tongue: English
• Leaves: 302
• Series: Methods in Molecular Biology 1801
• Edition: 1st ed.
• Category: Library

✦ Synopsis

This volume aims to detail the advances in the field of Thyroid hormones (THs), namely T3 and T4, and their nuclear receptor TRs from a conceptual and methodological point of view. Chapters guide readers through a general introduction, TR's engineered mice, human genetics of TRs, control of the hypothalamus-pituitary-thyroid (HPT) axis, the relevance of the local THs production and metabolism and pathological consequences of TH/TR alterations. Non-mammalian models are also presented and discussed. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Thyroid Hormone Nuclear Receptor: Methods and Protocols aims to ensure successful results in the further study of this vital field.

✦ Table of Contents

Front Matter ....Pages i-xii
Thyroid Hormone Receptors: Several Players for One Hormone and Multiple Functions (Jacques Samarut, Michelina Plateroti)....Pages 1-8
Two Protocols to Study the Interactions of Thyroid Hormone Receptors with Other Proteins and Chromatin (Wenyue Guan, Romain Guyot, Frédéric Flamant)....Pages 9-16
Expression Analysis of Genes Regulated by Thyroid Hormone in Neural Cells (Juan Bernal, Beatriz Morte)....Pages 17-28
In Vitro Approaches to Identify Thyroid Hormone Receptor-Dependent Transcriptional Response (Maria Sirakov, Michelina Plateroti)....Pages 29-38
Posttranslational Modification of Thyroid Hormone Nuclear Receptor by Phosphorylation (Yan-Yun Liu, Gregory A. Brent)....Pages 39-46
Posttranslational Modification of Thyroid Hormone Nuclear Receptor by Sumoylation (Yan-Yun Liu, Gregory A. Brent)....Pages 47-59
Demonstration of the Receptor Site for Thyroid Hormone on Integrin αvβ3 (Hung-Yun Lin, Shaker A. Mousa, Paul J. Davis)....Pages 61-65
The Deiodinase Trio and Thyroid Hormone Signaling (Antonio C. Bianco, Rodrigo R. da Conceição)....Pages 67-83
Thyroid Hormones and Derivatives: Endogenous Thyroid Hormones and Their Targets (Josef Köhrle)....Pages 85-104
Protocols for Studying How Thyroid Hormone Receptors Trigger Adaptive Thermogenesis in Mice (Karine Gauthier)....Pages 105-110
Pharmacological Inhibition of Lysosomal Activity as a Method For Monitoring Thyroid Hormone-induced Autophagic Flux in Mammalian Cells In Vitro (Jin Zhou, Rohit A. Sinha, Ronny Lesmana, Winifred W. Y. Yau, Paul M. Yen)....Pages 111-122
Analysis of Physiological Responses to Thyroid Hormones and Their Receptors in Bone (J. H. Duncan Bassett, Graham R. Williams)....Pages 123-154
Evaluating the Hypothalamic-Pituitary-Thyroid (HPT) Axis in Mice (F. E. Wondisford)....Pages 155-161
Thyroid Hormone Action: The p43 Mitochondrial Pathway (Chantal Wrutniak-Cabello, François Casas, Gérard Cabello)....Pages 163-181
Evaluating Thyroid Disrupting Chemicals In Vivo Using Xenopus laevis (Bilal B. Mughal, Barbara A. Demeneix, Jean-Baptiste Fini)....Pages 183-192
Quantification of Thyromimetic Sobetirome Concentration in Biological Tissue Samples (Jordan Devereaux, Skylar J. Ferrara, Thomas S. Scanlan)....Pages 193-206
Analysis of Thyroid Tumorigenesis in Xenograft Mouse Model (Xuguang Zhu, Sheue-Yann Cheng)....Pages 207-223
Human Genetics of Thyroid Hormone Receptor Beta: Resistance to Thyroid Hormone Beta (RTHβ) (Theodora Pappa, Samuel Refetoff)....Pages 225-240
TRα Mutations in Human (C. Briet, Natacha Bouhours-Nouet, F. Illouz, D. Prunier-Mirebeau, P. Rodien)....Pages 241-245
Role of Thyroid Hormone Receptor in Amphibian Development (Liezhen Fu, Luan Wen, Yun-Bo Shi)....Pages 247-263
De Novo Transcriptomic Approach to Study Thyroid Hormone Receptor Action in Non-mammalian Models (Nicolas Buisine, Gwenneg Kerdivel, Laurent M. Sachs)....Pages 265-285
Role of TRs in Zebrafish Development (Federica Marelli, Luca Persani)....Pages 287-298
Back Matter ....Pages 299-300

✦ Subjects

Biomedicine; Molecular Medicine