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Thymus colonization in the developing mouse embryo

✍ Scribed by Ronald Palacios; Jacqueline Samaridis

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
672 KB
Volume
21
Category
Article
ISSN
0014-2980

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✦ Synopsis

We have directly followed the formation of and the thymus colonization by pro-T lymphocytes in the developing C57BL/6 mouse embryo by using the monoclonal antibody JORO 37-5 specific for pro-T lymphocytes, immunofluorescence staining and flow fluorocytometry or microscopy analysis. The results show that JORO 37-5+ cells begin to appear in the liver at day 9 of gestation. These JORO 37-5+ cells migrate to and colonize the thymus 1 day later, where they expand vigorously during the next 4-5 days and, subsequently, switch off expression of JORO 37-5 as they further differentiate into mature thymocytes.

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