Thymidine kinase activity in synchronized hela cell cultures
β Scribed by Elton Stubblefield; Gerald C. Mueller
- Book ID
- 118849279
- Publisher
- Elsevier Science
- Year
- 1965
- Tongue
- English
- Weight
- 180 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0006-291X
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π SIMILAR VOLUMES
The deficiency in thymidine kinase activity of HeLa BU-100 cells was not due to negative feedback inhibition by high levels of BUdR or to interference with the thymidine kinase assay by inhibitors or competing enzymes in the HeLa BU-100 cell extracts. Followikg 5 weekly passages in nzedia lacking BU
Synchronous cultures of HeLa cells obtained by selective detachment of mitoses were treated with high concentrations of thymidine. The inhibitor was added soon after completion of cell division and the rates of cell enlargement and accumulation of DNA, RNA and protein were compared for untreated and