Thymic epithelium induces neither clonal deletion nor anergy to Mls 1a antigens
✍ Scribed by António Bandeira; António Coutinho; Odile Burlen-Defranoux; Ibrahim Khazaal; Monique Coltey; Frederic Jacquemart; Nicole Le Douarin; Josselyne Salaün
- Book ID
- 102822802
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 774 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
Thymic epithelium induces neither clonal deletion nor anergy to MIS la antigens*
Grafting of thymic anlagen from day-10 DBA/2 (H-2"; Mls-la) embryos to newborn athymic BALB/c (H-2"; Mls-lb) mice leads to reconstitution of T cell populations in the recipients. Analysis of adult chimeras shows that their Vfi T cell receptor (TcR) repertoires, particularly V76 and VpS.1, do not significantly differ in most animals (10 out of 13) from those scored in control chimeras that received syngeneic thymic anlagen. In all cases analyzed, such Mls-la-reactive T cells could be stimulated at levels comparable to control responses, both in vitro and in vivo.The few cases in which Mls-la reactivevp TcR were reduced seem to reflect the variability in TcR Vp repertoires found in this experimental system. In contrast, BALBk mice, injected at birth with DBA/2 spleen cells show a marked, albeit variable, reduction in the frequencies of V76and Vp8.l-bearing CD4+ T cells, and lower frequencies of Mls-l"-reactive T cells in limiting dilution analyses. It appears, however, that V76-and VoS. 1-bearing T cells remaining in these mice are functionally competent. We conclude that Mls-1 antigens are not expressed by thymic epithelium.