Thrombin potentiates the mitogenic response of cultured fibroblasts to serum and other growth promoting agents
✍ Scribed by Bruce R. Zetter; Tung-Tien Sun; Lan Bo Chen; John M. Buchanan
- Book ID
- 102309289
- Publisher
- John Wiley and Sons
- Year
- 1977
- Tongue
- English
- Weight
- 483 KB
- Volume
- 92
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Abstract
Thrombin may stimulate the proliferation of resting fibroblasts by itself or by potentiating the mitogenic effects of other growth stimulating agents. When added alone to a dense quiescent culture of chick embryo fibroblasts in either a serum‐free or a low serum medium, thrombin stimulates these cells to proliferation at a rate comparable to that seen with 5% serum. In the case of rabbit corneal fibroblasts neither fibroblast growth factor nor thrombin is particularly effective as a growth stimulant when added alone but exhibited a pronounced synergism on cell proliferation when present together. Established cell lines in the resting state, including 3T3, SV‐3T3, 3T6, BHK‐21 and 3T3 injected with avian sarcoma virus strain B77 (B77‐3T3), are not responsive to thrombin alone. When the serum concentration in the medium equals or exceeds 2%, thrombin potentiates the mitogenic response of 3T3 cells to serum factors. With the exception of 3T3 and SV3T3 cells, the other cell lines show a potentiation of growth when thrombin is added to a low‐serum (0.5%) medium containing epidermal growth factor and prostaglandin F~2α~. The addition of thrombin to cultures of B77‐3T3 cells growing in the presence of epidermal growth factor and prostaglandin F~2α~ does not increase the initial growth rate of these cells but increases significantly the final cell density of these cultures.