Thrombin chemotactic stimulation of HL-60 cells: Studies on thrombin responsiveness as a function of differentiation

✍ Scribed by Dr. R. Bar-Shavit; K. A. Hruska; A. J. Kahn; G. D. Wilner

• Publisher: John Wiley and Sons
• Year: 1987
• Tongue: English
• Weight: 730 KB
• Volume: 131
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041310216

✦ Synopsis

Thrombin, a major procoagulant enzyme and growth factor, i s also selectively chemotactic for monocytes and macrophages but not for neutrophils. This effect stands in contrast to other well-known chemotactic agents such as fMet-Leu-Phe, C5a fragments, and LTB4, which stimulate directed cell movement in both cell types, and have important physiological implications. The human leukemic cell line HL-60, which i s capable of differentiating either along granulocytic or monocytic lineages, was therefore used to explore the development of this selective monocytelmacrophage chemotactic response