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Three-phase partitioning for simultaneous renaturation and partial purification of Aspergillus niger xylanase

โœ Scribed by Ipsita Roy; Aparna Sharma; Munishwar Nath Gupta


Book ID
103823234
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
221 KB
Volume
1698
Category
Article
ISSN
1570-9639

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โœ Aparna Sharma; M. N. Gupta ๐Ÿ“‚ Article ๐Ÿ“… 2002 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 118 KB

It is shown that eudragit S-100, a copolymer of methylacrylic acid and methylmethacrylate, undergoes three-phase partitioning. It was found that 95% eudragit S-100 could be recovered as the interfacial precipitate by using 30% (w/v) ammonium sulfate, 1:1 ratio of t-butanol to polymer solution at 40