✦ LIBER ✦
Three Novel Spin-Labeled Substrates for Enzymatic Incorporation into Nucleic Acid Lattices
✍ Scribed by Charles R. Toppin; Gary T. Pauly; Prabu Devanesan; David Kryak; Albert M. Bobst
- Book ID
- 102256084
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- German
- Weight
- 322 KB
- Volume
- 69
- Category
- Article
- ISSN
- 0018-019X
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✦ Synopsis
The synthesis of one C(4)-spin-laheled uridine-S'-diphosphate (C(4)-UDP and two nitroxide-containing 2'-deoxyuridine-S'-triphosphate (dUTP) analogs are reported. The C(4)-UDP derivative was incorporated into copolymers by polynucleotide phosphoxylase (PNPase); one of' the two dUTP analogs, substituted at position C(4), was a good substrate for TdT, whereas the other one, substituted at position C(5), served as substrate for E. coii DNA polymerase (Pol 1).