Three distinct commonly deleted regions of chromosome arm 16q in human primary and metastatic prostate cancers

✍ Scribed by Hiroyoshi Suzuki; Akira Komiya; Mitsuru Emi; Hiroaki Kuramochi; Taizo Shiraishi; Ryuichi Yatani; Jun Shimazaki

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 752 KB
• Volume: 17
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/(sici)1098-2264(199612)17:4<225::aid-gcc4>3.0.co;2-5

✦ Synopsis

Human prostate cancers frequently show loss of heterozygosity (LOH) at loci on the long arm of chromosome I 6 (I 6q). In this study, we analyzed prostate cancer specimens from 48 patients (Stage B, 20 cases; Stage C, I0 cases; cancer death, I 8 cases) for allelic loss on I6q, using either restriction fragment length polymorphism (RFLP)-or polymerase chain reaction (PCR)-based methods. Allelic losses were observed in 20 (42%) of 48 cases, all of which were informative with at least one locus. Detailed deletion mapping identified three distinct commonly deleted regions on this chromosome arm: q22. I -q22.3, q23.2-q24. I, and q24.3-qter. On the basis of a published sex-averaged framework map, the estimated sizes of the commonly deleted regions were 4.7 (I 6q22. I -q22.3), 17.2 (I 6q23.2-q24. I) and 8.4 cM (16q24.3-qter). Allelic losses on 16q were observed more frequently in the cancer-death cases ( I I of I 8 6 I %) than in early-stage tumor cases (9 of 30; 30%. P < 0.05). In 7 of I I patients from whom DNA was available from metastatic cancers as well as from normal tissues and primary tumors, the primary cancer foci had no detectable abnormality of I6q, but the metastatic tumors showed LOH. These results suggest that inactivation of tumor suppressor genes on 16q plays an important role in the progression of prostate cancer. We also analyzed exons 5-8 of the E-cadherin gene, located at I6q22. I, in tumor DNA by means of PCR-single strand conformation polymorphism and direct sequencing, but we detected no somatic mutations in this candidate gene.