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Three-dimensional1H-TOCSY-relayed ct-[13C,1H]-HMQC for aromatic spin system identification in uniformly13C-labeled proteins

✍ Scribed by Oliver Zerbe; Thomas Szyperski; Marcel Ottiger; Kurt Wüthrich

Book ID
104662448
Publisher
Springer Netherlands
Year
1996
Tongue
English
Weight
751 KB
Volume
7
Category
Article
ISSN
0925-2738

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✦ Synopsis

Three-dimensional IH-TOCSY-relayed ct-[~3C, IH]-HMQC is a novel experiment for aromatic spin system identification in uniformly ~3C-labeled proteins, which is implemented so that it correlates the chemical shift of a given aromatic proton with those of the directly attached carbon and all vicinal protons. The ct-HMQC scheme is used both for overlay of the indirect IH and 13C chemical shift evolution periods and for the generation of JH-~H antiphase magnetization to accelerate the ~H-TOCSY magnetization transfer at short mixing times. As an illustration, data recorded for the 18 kDa protein cyclophilin A are presented. Since transverse relaxation of J3C-~H zero-quantum and double-quantum coherences is to first order insensitive to ~3C-~H heteronuclear dipolar relaxation, the new experiment should work also for proteins with molecular weights above 20 kDa.

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