Three-dimensional reconstruction from serial sections. I. A portable microcomputer-based software package in Fortran

✍ Scribed by Jeffrey Prothero; John Prothero

• Publisher: Elsevier Science
• Year: 1982
• Tongue: English
• Weight: 452 KB
• Volume: 15
• Category: Article
• ISSN: 0010-4809
• DOI: 10.1016/0010-4809(82)90021-0

✦ Synopsis

A software package is described that is designed to simplify the collection, storage, and processing of data to be used for three-dimensional reconstruction from serial sections. The package, called MORPHO, consists of four independent programs, all written in FOR-TRAN, running under CP/M, and called ISPY, DMPSPY, EDSPY, and STATSPY. The function of ISPY is to guide the user in the documentation, digitization, and storage of data derived from serial section images. The serial sections may be at any level of resolution: ultrastructural, microscopic, or macroscopic. DMPSPY allows the user to display and to make hard copies of the digitized contours. EDSPY allows any and all errors in data entry to be rectified simply and directly. STATSPY carries out statistical calculations, such as crosssectional areas and volumes. A useful feature of STATSPY is the provision for easy insertion of additional subroutines to compute special statistics not currently provided.