Three-dimensional morphometrical study of dendritic spines of the granule cell in the rat dentate gyrus with HVEM stereo images
✍ Scribed by Hama, Kiyoshi ;Arii, Tatsuo ;Kosaka, Toshio
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1989
- Tongue
- English
- Weight
- 806 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0741-0581
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✦ Synopsis
Kurnber, length, and diameters of dendritic spines of the granule cell in the dorsal leaf of the rat dentate gyrus were measured by using high-voltage electron microscope stereo images of 5-pm-thick Golgi preparations with the aid of a three-dimensional image analyzer system.
Spine densities of 2.02 2 0.28, 2.28 f 0.33, and 3.36-t 0.35 per 1 pm at distal, middle, and proximal portions of the dendrite were obtained. These values were about 1.6-fold of the previous light microscopical report.
Mean three-dimensional spine length were 1.244 * 0.506 pm, 1.262 * 0.563 pm, and 1.254 i : 0.584 pm at distal, middle, and proximal portions, respectively, which were about 1.4 times longer than those measured in two dimensions.
By using measured morphometrical parameters of spines such as lengths, diameters, and population densities, total spine surface areas of 2.401 p.m2, 2.806 pmz, and 4.180 pm2 per 1 p. m of the dendrite at distal, middle, and proximal portions, respectively, were obtained. The total surface area of dendrite was about doubled by the addition of the spines at each dendritic portion.
3D image analysis, High voltage electron microscope
The advantageous features and the problems of the present method are discussed.