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Three-Dimensional Elution Mapping of Pyridylaminated N-Linked Neutral and Sialyl Oligosaccharides

โœ Scribed by N. Takahashi; H. Nakagawa; K. Fujikawa; Y. Kawamura; N. Tomiya

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
484 KB
Volume
226
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

We propose a three-dimensional (3-D) sugar-mapping technique for pyridylaminated (PA) neutral and sialyl oligosaccharides as a powerful structural characterization of (\mathrm{N})-linked oligosaccharides using only picomoles of samples. The new map consists of the elution data from 42 different sialyl oligosacharides, 26 of which are mono-, 7 of which are di-, 7 of which are tri-, and 2 of which are tetra-sialylated oligosaccharides. The 20 standard sialyl oligosaccharides were released from human serum and calf fetuin by digestion with glycoamidase (A). The other 22 standard sialyl oligosaccharides were obtained by subsequent digestion of the above 20 sialyl oligosaccharides with (\beta)-galactosidase, (\beta) - (\boldsymbol{N})-acetylhexosaminidase, (\alpha)-fucosidase, and (\alpha 2 \rightarrow 3) specific sialidase.

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