Three-Dimensional Electron Microscopy of Macromolecular Assemblies

✍ Scribed by Joachim Frank

• Publisher: Academic Press
• Year: 1996
• Tongue: English
• Leaves: 361
• Edition: 1
• Category: Library

✦ Synopsis

This concise and easy to read book is essentialy a crash course in single particle reconstruction by electron microscopy. It isn't exactly a how-to manual, more an overview of how the whole process works. Thus, it is useful not only to someone who wants to learn how to do this, but also to those who might be interested in deciding if this technique would be useful to them. And actually, the whole topic is sufficiently cool that I would recommend the book even to people who know they will never use this technique, just because its fun to read about it. The book is clearly written and doesn't assume alot of prior knowledge. A basic undergrad education in math would be enough to handle the equations.

✦ Table of Contents

Front Cover......Page 1
Three-Dimensional Electron Microscopy of Macromolecular Assemblies......Page 4
Copyright Page......Page 5
Contents......Page 8
Preface......Page 16
I. The Electron Microscope and Biology......Page 20
II. Single-Particle versus Crystallographic Analysis......Page 25
III. Crystallography without Crystals......Page 26
IV. Toward a Unified Approach to Structure Research......Page 28
V. The Electron Microscope and the Computer......Page 29
I. Specimen Preparation Methods......Page 31
II. Principle of Image Formation in the Electron Microscope......Page 43
III. Special Imaging Techniques......Page 68
I. Introduction......Page 73
II. Digitization and Selection of Particles......Page 86
III. Alignment Methods......Page 92
IV. Averaging and Global Variance Analysis......Page 120
V. Resolution......Page 129
VI. Validation of the Average Image: Rank Sum Analysis......Page 142
VII. Outlier Rejection: "Odd Men Out" Strategy......Page 143
I. Introduction......Page 145
II. Theory of Correspondence Analysis......Page 154
III. Correspondence Analysis in Practice......Page 164
IV. Classification......Page 179
I. Introduction......Page 201
II. General Mathematical Principles......Page 202
III. Rationales of Data Collection: Reconstruction Schemes......Page 207
IV. Overview of Existing Reconstruction Techniques......Page 221
V. The Random Conical Reconstruction Scheme in Practice......Page 230
VI. Merging of Reconstructions......Page 244
VII. Three-Dimensional Restoration......Page 250
VIII. Angular Refinement Techniques......Page 254
IX. Transfer Function Correction......Page 264
I. Preliminaries: Significance, Experimental Validity, and Meaning......Page 266
II. Assessment of Statistical Significance......Page 267
III. Validation and Consistency......Page 273
IV. Visualization and Segmentation......Page 279
V. Juxtaposition with Existing Knowledge......Page 287
I. Introduction......Page 292
II. Image Processing and Three Dimensional Reconstruction of the Calcium Release Channel......Page 294
II. Basic Design Features......Page 301
Ill. Existing Packages......Page 304
IV. Interfacing to Other Software......Page 306
V. Documentation......Page 307
Appendix 2: Macromolecular Assemblies Reconstructed from Images of Single Macromolecules......Page 308
Bibliography......Page 312
Index......Page 352