Three-dimensional culture for expansion and differentiation of mouse embryonic stem cells
β Scribed by Hui Liu; Scott F. Collins; Laura J. Suggs
- Book ID
- 108069721
- Publisher
- Elsevier Science
- Year
- 2006
- Tongue
- English
- Weight
- 624 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0142-9612
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Several groups have demonstrated the existence of selfrenewing stem cells in embryonic and adult mouse brain. In vitro, these cells proliferate in response to epidermal growth factor, forming clusters of nestin-positive cells that may be dissociated and subcultured repetitively. Here we show that,