Three-Dimensional 13C-Spectroscopic Imaging in the Isolated Infarcted Rat Heart

✍ Scribed by Claudia Weidensteiner; Titus Lanz; Michael Horn; Stefan Neubauer; Axel Haase; Markus von Kienlin

• Publisher: Elsevier Science
• Year: 2000
• Tongue: English
• Weight: 269 KB
• Volume: 143
• Category: Article
• ISSN: 1090-7807
• DOI: 10.1006/jmre.1999.1984

✦ Synopsis

Acquisition weighted (13)C-spectroscopic imaging with three spatial dimensions is demonstrated in the isolated, perfused rat heart. Experiments were performed at 11.75 T with a home-built double resonant (13)C-(1)H probehead. Three-dimensional chemical shift imaging was used to obtain (1)H-decoupled (13)C-spectra in 96-microl voxels in about 58 min. Acquisition weighting significantly reduced signal contamination and improved image quality, with no penalty in sensitivity. As a first application, infarcted hearts were studied during perfusion with [2-(13)C]-sodium acetate. The extent of the incorporation of the (13)C-label into glutamate allows us to distinguish intact and infarcted myocardium. Chemical shift images show a homogeneous glutamate distribution in intact tissue, but a negligible amount in the infarction scar.