𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Three- and four-dimensional visualization of cell migration using optical coherence tomography

✍ Scribed by Sara M. Rey; Boris Považay; Bernd Hofer; Angelika Unterhuber; Boris Hermann; Adrian Harwood; Wolfgang Drexler

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
535 KB
Volume
2
Category
Article
ISSN
1864-063X

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✦ Synopsis

Abstract

Conventionally, cell chemotaxis is studied on two‐dimensional (2D) transparent surfaces, due to limitations in optical and image data‐collection techniques. However, surfaces that more closely mimic the natural environment of cells are often opaque. Optical coherence tomography (OCT) is a noninvasive label‐free imaging technique, which offers the potential to visualize moving cells on opaque surfaces and in three dimensions (3D). Here, we demonstrate that OCT is an effective means of time‐lapse videomicroscopy of Dictyostelium cells undergoing 3D (2D+time) cell migration on nitrocellulose substrates and 4D (3D+time) chemotaxis within low‐density agarose gels. The generated image sequences are compatible with current computer‐based image‐analysis software for quantification of cell motility. This demonstrates the utility of OCT for cell tracking and analysis of cell chemotaxis in complex environments. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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