Thiophilic gels: applications in flow-injection immunoassays for macromolecules and haptens

✍ Scribed by Derek A. Palmer; James N. Miller

• Book ID: 102985737
• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 654 KB
• Volume: 303
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(94)00536-u

✦ Synopsis

Simple fluorescence flow-injection immunoassays have been developed for theophylline (a hapten) and albumin (a macromolecule) using a thiophilic affinity matrix (T-gel). This gel is far superior to the previously used bacterial immunoglobulin binding proteins (e.g., proteins A and G) in terms of cost, elution conditions, non-specific binding and specificity for immunoglobulins regardless of type or subclass. Both preincubation and on-line immunoassay formats were investigated. Human serum albumin and theophylline were determined by this method and good R.S.D. and %recoveries were achieved.