Thionucleoside disulfides as covalent constraints of DNA conformation

✍ Scribed by Robert S. Coleman; Jason L. McCary; Ronelito J. Perez

• Book ID: 104209620
• Publisher: Elsevier Science
• Year: 1999
• Tongue: French
• Weight: 805 KB
• Volume: 55
• Category: Article
• ISSN: 0040-4020
• DOI: 10.1016/s0040-4020(99)00704-8

✦ Synopsis

Full details of a new strategy for cross-linking of duplex oligonucleotides are described. A direct base-to-base disulfide bond is formed by oxidation of two appropriately positioned thionucleoside bases. Cross-link formation between 4-thio-2'-deoxyuridine and 6-thio-2'-deoxyinosine is demonstrated in three sequence contexts (3' to 3', 5' to 5', and opposed). Disulfide formation occurs in high yield in both duplex and hairpin DNA. Molecular modeling and circular dichroism were used to demonstrate minimal distortion of the constrained B-DNA double helix.