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Thiol group determination in proteins by flameless atomic absorption spectrometry of mercury

✍ Scribed by Jens B. Carlsen

Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
320 KB
Volume
64
Category
Article
ISSN
0003-2697

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✦ Synopsis

The flameless atomic absorption procedure for determination of minute amount of mercury has been adapted for measuring thiol-groups in native and denatured proteins. The excess of p-hydroxymercuribenzoate is removed by gel filtration after its reaction with the protein. The protein-p-hydroxymercuribenzoate complex is digested and the mercury is determined in an atomic absorption spectrophotometer. The sensitivity of the method allows measurement of 0.05 ~zg protein-bound mercury with the equipment used, but can be further increased. Results are presented for five different pl-oteins.

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