Characterization of thiol-, aspartyl-, a
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Vitor Oliveira; Emer S. Ferro; Marcelo D. Gomes; Maria E.M. Oshiro; Paulo C. Alm
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Article
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2000
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John Wiley and Sons
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English
โ 228 KB
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We combined fluorogenic substrates or internally quenched fluorescent peptides with specific inhibitors in the pH profile of proteolytic activity experiments in order to detect proteolytic activities in lysates of MDCK cells. Hydrolytic activities related to cathepsin B, L, and D were observed. Seri