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Thioacylating intermediates as metabolites of S-(1,2-dichlorovinyl)-L-cysteine and S-(1,2,2-trichlorovinyl)-L-cysteine formed by cysteine conjugate .beta.-lyase

✍ Scribed by Dekant, Wolfgang; Berthold, Klemens; Vamvakas, Spyridon; Henschler, Dietrich; Anders, M. W.


Book ID
127342569
Publisher
American Chemical Society
Year
1988
Tongue
English
Weight
529 KB
Volume
1
Category
Article
ISSN
0893-228X

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High-performance liquid chromatography-f
✍ Gerard J. Stijntjes; Johan M. te Koppele; Nico P.E. Vermeulen πŸ“‚ Article πŸ“… 1992 πŸ› Elsevier Science 🌐 English βš– 936 KB

An HPLC-fluorescence assay has been developed for the determination of the activity of rat renal cytosolic cysteine conjugate beta-lyase. The method is based on isocratic HPLC separation and fluorescence detection of pyruvic acid, derivatized with o-phenylenediamine (OPD), and is shown to be rapid,