Thin-layer liquid crystal thermometry of cells in vitro during hyperthermal microwave irradiation
✍ Scribed by J. Eugene Robinson; Duncan McCulloch; George H. Harrison; Augustine Y. Cheung
- Book ID
- 101707757
- Publisher
- John Wiley and Sons
- Year
- 1982
- Tongue
- English
- Weight
- 492 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0197-8462
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
A nonperturbing technique of thin‐layer liquid crystal thermometry was developed to quantitate heating of Chinese hamster ovary cells and the bacterium Serratia marcescens when exposed to 2450‐MHz microwave fields at 0.2–0.5 W/cm^2^. Cells suspended in culture medium were injected into 5‐cm glass microcapillary tubes coated on the inside with a thin layer of liquid crystal. The tubes were sealed and placed parallel to the electric field in a watertight waveguide exposure chamber where they were heated by circulating temperature‐controlled water. Even at high circulation rates, liquid crystal color changes indicated local microwave capillary tube heating of 0.1–0.25 °C. Precision of measurement was 0.02 °C. Observations during microwave heating were significantly different from observations without microwaves at the 1% level, and heating increased as circulating water flow was reduced from 300 ml/s to 100 ml/s. The results of a cell survival assay following hyperthermal treatment were in good agreement with expectations based on the observations of microwave heating using liquid crystals.