Thin-layer detection of diazepam and/or chlordiazepoxide alone or in combination with major drugs of abuse in drug abuse urine screening programs

✍ Scribed by K.K. Kaistha; R. Tadrus

• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 701 KB
• Volume: 154
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)98462-0

✦ Synopsis

Three extraction procedures for the detection of diazepam, oxazepam, chlorazepate and/or chlordiazepoxide in human urines are presented. All three procedures are based on the acid hydrolysis of benzodiazepines and/or their conjugated metabelites to give the corresponding benzophenones. Procedure I involves the direct acid hydrolysis of raw urine and is recommended when the aim is to test the abuse of benzodiszepine derivatives only. Procedure 11 is a two-step extraction method in which a wide variety of drugs of abuse including cocaine (test based OQ the detection of benzoylecgonine) are extracted by the first step using paper loaded with cationexchange resin and the benzodiazepines are tested in the second step by the acid hydrolysis of the spent urine left after removing the ion-exchange paper. Procedure IH involves the use of inert fibrous matrix and then its acid hydrolysis. The detection procedure is based on the identifkation of methylaminochlorobenzophenone (MACB) and aminochlorobenzophenone (ACB). MAC73 is detected as a yellow-colored compound while ACB is detected by spraying with Bratton-Marshall reagent. Speciikity of detection of ACB has been achieved by the selection of a thin-layer developing solvent system in which sulfonamides with primary aromatic amino groups remain at the origin. * Reptits avaikble at USS I.50 each.