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Thin-layer chromatography of β-aminopropionitrile

✍ Scribed by Joseph H. Fleisher; Klaus Brendel; Milos Chvapil; Erle E. Peacock

Publisher: Elsevier Science
Year: 1976
Tongue: English
Weight: 337 KB
Volume: 74
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(76)90331-6

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✦ Synopsis

Thin-Layer Chromatography of P-Aminopropionitrilel Thin-layer chromatography of p-aminopropionitrile (BAPN) in acetone and 1 M ammonium hydroxide (9:l) allowed separation of that compound from amino acids present in rat-liver perfusion fluid without prior solvent extraction. Direct densitometry of the spots obtained with ninhydrin yielded satisfactory quantitation of P-aminopropionitrile present. Utilization of [14C]nitrile-labeled /?-aminopropionitrile and concurrent analysis of cyanoacetic acid allowed almost complete accountability of BAPN added to isolated rat liver.

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