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Thin-layer chromatography of rat bile and urine following intravenous administration of the pesticidal synergist octachlorodipropyl ether

✍ Scribed by L. Fishbein; J. Fawkes; H.L. Falk; S. Jordan

Book ID: 104147343
Publisher: Elsevier Science
Year: 1968
Tongue: English
Weight: 664 KB
Volume: 37
Category: Article
ISSN: 1873-3778
DOI: 10.1016/s0021-9673(01)99103-4

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✦ Synopsis

Chromatographic differences, in rat bile and urine samples resulting from single intravenous administration of octachlorodipropyl ether were elaborated using Silica Gel DF-5 chromatoplates with toluene-acetic acid-water (IO : IO : I) as developer. Detection was accomplished by 2537 A and 36Go A ultraviolet as tie11 as cont. sulfuric acid-rt-butanol and silver nitrate-2 N alcoholic potassium hydroxide chromogenic reagents. Ten metabolites were detected in the bile and, 5 in the urine. The relative rates of elimination of the major biliary and urinary metabolites were compared.

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Bile and urine samples were applied with a RADIN-PELIDS thin-layer sample streaker obtained from Applied Science Laboratories, State College, Pa., U.S.A. \*\* Obtained from Camag, Muttonz, Switzerland. \*\* \* Two thin-layer glass plates were used to coyor the areas that were not to receive the spra