Thin-Layer Chromatography of Nucleotides

✍ Scribed by Dr. K. Randerath

• Publisher: John Wiley and Sons
• Year: 1962
• Tongue: English
• Weight: 500 KB
• Volume: 1
• Category: Article
• ISSN: 0044-8249
• DOI: 10.1002/anie.196204351

✦ Synopsis

Abstract

Thin‐layer chromatography of nucleotides on DEAE‐ and ECTEOLA‐cellulose is superior to the methods of seperation in use up to the present. Using dilute hydrochloric acid, and without a gradient elution technique, it is possinle to acheive a group seperation of monodi‐, and tri‐phosphates as well as subfractionation within these groups depending on the basicity of the nucleobases. Thin‐layer chromatography is appreciably more sensitive than paper chromatography; 5×10^−4^ mole adenine neuclotides can be still be detected. The total quantity of material that can be chromatographed without tailing is greater in this procedure than that with paper chromatography; a mixture consisting of 1 μmole ATP and ADP per starting spot can be resolved with ease . Hence the procedure can also be applied for micro‐scale preparative seperations . A mixtures of 10^−2^ μmole ADP and ATP, respectively, can be seperated completely in four to ten minutes.