𝔖 Bobbio Scriptorium
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Thin-layer chromatography of free porphyrins on talc

✍ Scribed by T.K. With


Book ID
108313688
Publisher
Elsevier Science
Year
1967
Tongue
English
Weight
11 KB
Volume
1
Category
Article
ISSN
0009-9120

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Mixtures of protoporphyrin, deuteroporphyrin, haematoporphyrin, pemptoporphyrin and mesoporphyrin can be separated as free acids on talc thin-layer chromatography plates by developing them with a mixture of ethanol-2,6-lutidine-water (30:3:67) in tanks containing an atmosphere saturated with ammonia

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After separating porphyrins as their metllyl esters .by .Gilica gel rtlhb-!layar chromatograpliy, in the analytical range, a. complementary fluoromekic m;leffhod ma5 developed for quantitative iqz sit?4 fluorometry of porphyrin tllin-layeri~l~on;l~~~~sunns. Commercial silica gel layers with an alumi