Thin-layer chromatographic fluorimetry of indole derivatives after condensation by a paraformaldehyde spray reagent

✍ Scribed by Mark I. Toneby

• Book ID: 104146578
• Publisher: Elsevier Science
• Year: 1974
• Tongue: English
• Weight: 669 KB
• Volume: 97
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)97583-1

✦ Synopsis

A sensitive ptlrt~f~~rmafdeh~de spray reagent for the thin-layer chromato~raphic detection of indofe derivatives at the nanogram level is described_ Paraformafdehyde is dissolved in sfightl_v alkaline etfmnof and the ethnnofic solution is neutralized by addition of acetic acid. The tluorescence is measured spectrophotofluorin~etric~fl~ on tRin-fa:er cfimnatogran~s and observations are made visually and on photogizlphic lilrns esposed through filters_ Excitation and emission spectra are given for nine indofe derivatives reacted \vith the part~formtlldef~~de spray reagent.