Thin-layer chromatographic analysis of enzymic hydrolysates of agar

✍ Scribed by M. Duckworth; W. Yaphe

• Publisher: Elsevier Science
• Year: 1970
• Tongue: English
• Weight: 511 KB
• Volume: 49
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)93663-x

✦ Synopsis

The most suitable solvent systems for the analysis, by cellulose thin-layer chromatography of the neutral and charged oligosaccharides formed by the enzymic hydrolysis of agar have been evaluated. As the mixture of oligosaccharides is not adequately resolved by one or two dimensional thin-layer chromatography to enable identification of the individual components, the neutral and charged sugars are first separated from each other on DEAE-Sephadex A-25 (Cl-). The solvent system for the optimum separation of the neutral sugars depends on the degree of polymerisation of the oligosaccharides under investigation.

A good separation of the charged oligosaccharides is obtained in the solvent system butan-I-ol-acetic acid-water (4: I : 2).