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Thin Layer and Column Chromatography of Carbohydrates as Trimethylsilyl Ethers with Applications to Mucopolysaccharide Analysis.

โœ Scribed by Karkkainen, J. E.; Haahti, E. O.; Lehionen, A. A.


Book ID
125994439
Publisher
American Chemical Society
Year
1966
Tongue
English
Weight
455 KB
Volume
38
Category
Article
ISSN
0003-2700

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A method was developed to extract quantitatively the bilirubins from bile, urine, serum, stool, and preparations from liver with a chloroform-ethanol mixture at pH 1.8 in the presence of ascorbic acid and NaC1. Extracted pigment was submitted to thin-layer chromatography, and the separated bilirubin