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Thiamine-binding protein and thiamine uptake by Escherichia coli

โœ Scribed by Nishume, Takahiro; Hayashi, Ryoji


Book ID
122034753
Publisher
Elsevier Science
Year
1971
Tongue
English
Weight
600 KB
Volume
244
Category
Article
ISSN
0304-4165

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A rapid assay procedure for thiamine-bin
โœ Takahiro Nishimune; Ryoji Hayashi ๐Ÿ“‚ Article ๐Ÿ“… 1973 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 275 KB

Although there are many methods available for detecting ligand binding to macromolecules (l-5), equilibrium dialysis is still the most commonly used. We report here a simple variation on equilibrium dialysis that greatly shortens the time needed for the assay of the thiaminebinding protein of E. col