Thiamin transport by human erythrocytes and ghosts
β Scribed by D. Casirola; C. Patrini; G. Ferrari; G. Rindi
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 703 KB
- Volume
- 118
- Category
- Article
- ISSN
- 0022-2631
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π SIMILAR VOLUMES
## Abstract The inhibition of glucose transport by the nonβionizable localβanesthetic benzylalcohol is of the mixed type and independent of pH. The affinity of benzylalcohol to the free carrier is about three times larger than that to the carrierβglucose complex.
Human erythrocyte transketolase could be resolved from thiamin diphosphate (TDP) by acidification of the ammonium sulfate precipitate to pH 3.5, but not by other tested procedures. Resolution was 98% by chemical measurement of residual thiamin and 95% by residual enzyme activity. Reconstitution of t