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Thiamin-Diphosphate-Dependent Enzymes: New Aspects of Asymmetric CC Bond Formation

✍ Scribed by Martina Pohl; Bettina Lingen; Michael Müller


Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
224 KB
Volume
8
Category
Article
ISSN
0947-6539

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✍ Dörte Gocke; Cong Luan Nguyen; Martina Pohl; Thomas Stillger; Lydia Walter; Mich 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 164 KB 👁 1 views

## Abstract The thiamine diphosphate‐dependent, branched‐chain 2‐keto acid decarboxylase from __Lactococcus lactis__ sup. __cremoris__ B1157 (KdcA) is a new valuable enzyme for the synthesis of chiral 2‐hydroxy ketones. The gene was cloned and the enzyme was expressed as an N‐terminal hexahistidine