Thermostable extracellular protease ofBacillus stearothermophilus: factors affecting its production

✍ Scribed by N. A. Razak; M. Y. A. Samad; M. Basri; W. M. Z. W. Yunus; K. Ampon; A. B. Salleh

• Book ID: 104764943
• Publisher: Springer
• Year: 1994
• Tongue: English
• Weight: 284 KB
• Volume: 10
• Category: Article
• ISSN: 1573-0972
• DOI: 10.1007/bf00414858

✦ Synopsis

A strain of protease-producing Bacillus stearothermophilus has been isolated. Glycerol was the best carbon source for production whereas yeast extract was the best nitrogen source. The bacterium could grow up to 70°C but optimum protease production was at 60°C. Best initial pH for protease production was 5. Alkaline pH inhibited production. The enzyme was stable at 60°C for 18 h and was inhibited by EDTA, PMSF and HgCl2.