Thermostabilization of enzymes by the chaperonin GroEL

✍ Scribed by J. A. Mendoza; M. Manson; F. Joves; E. Ackermann

• Book ID: 104648699
• Publisher: Springer-Verlag
• Year: 1996
• Tongue: English
• Weight: 373 KB
• Volume: 10
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00159520

✦ Synopsis

The influence of GroEL on the heat-inactivation of nine enzymes was analyzed. Five dehydrogenases and four other unrelated enzymes were heat-inactivated in the absence and presence of GroEL, at three different temperatures. GroEL protected most enzymes against inactivation and prevented their aggregation.

Further, the formation of a highly stable complex was observed when rhodanese was thermoinactivated in the presence of GroEL.