Thermospray high performance liquid chromatographic/mass spectrometric analysis of some fusarium mycotoxins

✍ Scribed by Voyksner, R. D. ;Hagler, W. M. ;Tyczkowska, K. ;Haney, C. A.

• Publisher: John Wiley and Sons
• Year: 1985
• Tongue: English
• Weight: 481 KB
• Volume: 8
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240080303

✦ Synopsis

Thermospray high performance liquid chromatography/mass spectrometry (TSP HPLC/MS) was used to analyrefiveFusarium mycotoxins in porcine plasma and urine. Four cytotoxic trichothecene mycotoxins, T-2 toxin, HT-2 toxin, diacetoxyscirpenol (DAS),deoxynivalenol (DON),T2tetraol,and the fungalestrogen zearalenone (F-2 toxin) were analyzed. The thermospray mass spectrum contained molecular weight information with few, if any, fragment signals. Detection limits rangingfrom 1 to 10 ngof mycotoxin injected onto the HPLC column were obtained using selected ion monitoring (SIM) HPLCIMS. Neither the plasma nor the urine matrix interfered with TSP HPLC/MS analysis of these mycotoxins and no sample derivatization was necessary for the analysis. The TSP HPLC/MS technique appears to be ideal for very sensitive analysis of mycotoxins in biological samples.