Thermooptical flow-injection determination for hydrogen peroxide based on an enzymic reaction heat-induced optical beam deflection

✍ Scribed by Xing-Zheng Wu; Hiroaki Shindoh; Toshiyuki Hobo

• Book ID: 102624010
• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 353 KB
• Volume: 299
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(94)00465-x

✦ Synopsis

A novel thetmooptical flow-injection method for hydrogen peroxide determination is proposed, based on immobilized enzymic (catalase) reaction heat-induced optical beam deflection. A glass tube, whose one end is attached with a thin gold film on which the catalase is immobilized, is used as a reaction cell. The reaction cell is immersed into a CCl, phase where a probe beam is passed through. Hydrogen peroxide solution is injected into the stream of sodium phosphate buffer solution (pH 7.4) flowing into the reaction cell. The reaction heat of the decomposition reaction of H,O, catalyzed by the immobilized catalaae induces a deflection of the probe beam. The immobilization of catalase by crosslinking with bovine serum albumin is optimized to obtain a maximum beam deflection signal. The deflection signal is linear over the concentration range of 0.025-0.5 mol 1-l (40 ~1 injected) for H,O, and the detection limit is ca. 0.025 mol 1-l. This thermooptical flow-injection detection system has good stability and reproducibility. The noise source is discussed and the improvements on the experimental setup are also discussed.