Thermoluminescence properties of the isolated photosystem two reaction centre
โ Scribed by Imre Vass; David J. Chapman; James Barber
- Book ID
- 104623251
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 512 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0166-8595
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โฆ Synopsis
Formation of thermoluminescence signals is characteristic of energy-and charge storage in Photosystem II. In isolated D1/D2/cytochrome b-559 Photosystem II reaction centre preparation four thermoluminescence components were found. These appear at -180 (Z band), between -80 and -50 (Zv band), at -30 and at + 35ยฐC. The Z band arises from pigment molecules but not correlated with photosynthetic activity. The Zv and -30ยฐC bands arise from the recombination of charge pairs stabilized in the Photosystem II reaction centre complex. The + 35ยฐC band probably corresponds to the artefact glow peak resulting from a pigment-protein-detergent interaction in subchloroplast preparations (R6zsa Zs, Droppa M and Horv/tth G (1989) Biochim Biophys Acta 973, 350-353).
๐ SIMILAR VOLUMES
A rapid procedure has been developed for the isolation of the photosystem two reaction centre complex (PS II RC) from a double mutant of Chlamydomonas reinhardtii, F54-14, which lacks the Photosystem one complex and the chloroplast ATPase. Thylakoid membranes are solubilised with 1.5% (w/v) Triton X