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Thermodynamics of the Unfolding and Spectroscopic Properties of the V66W Mutant of Staphylococcal Nuclease and Its 1−136 Fragment †

✍ Scribed by Eftink, Maurice R.; Ionescu, Roxana; Ramsay, Glen D.; Wong, Cing-Yuen; Wu, Jie Q.; Maki, August H.

Book ID
126187596
Publisher
American Chemical Society
Year
1996
Tongue
English
Weight
460 KB
Volume
35
Category
Article
ISSN
0006-2960

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Fluorescence and circular dichroism data as a function of temperature were obtained to characterize the unfolding of nuclease A and two of its less stable mutants. These spectroscopic data were obtained with a modified instrument that enables the nearly simultaneous detection of both fluorescence an