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Thermodynamics of polymolecular duplexes between phosphate-methylated DNA and natural DNA

✍ Scribed by Maurice R. Moody; Marcel H. P. van Genderen; Henk M. Buck

Publisher
Wiley (John Wiley & Sons)
Year
1990
Tongue
English
Weight
784 KB
Volume
30
Category
Article
ISSN
0006-3525

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✦ Synopsis

MB Eindhoven, T h e Netherlands

SYNOPSIS

Phosphate-methylated ( P.M.) DNA possesses a very high affinity for complementary natural DNA, as a result of the absence of interstrand electrostatic repulsions. In this study, a model system phosphate-methylated d [ C,] with natural d ( G k ) ( n < 12) is chosen for an investigation of the thermodynamic properties that determine duplex stability. The enthalpy change of a melting transition is shown to be considerably larger than is observed for corresponding natural DNA duplexes. It is found that AH: of G G / C C nearest neighbor pairwise interaction equals -15.6 kcal/mol, compared to -11.0 kcal/mol for the natural analog. The entropy change is strongly dependent on the length of the natural DNA strand and the number of phosphate-methylated DNA oligomers hybridized. The results are explained by means of a model in which a cooperative effect for subsequent hybridizations of phosphate-methylated DNA oligomers is assumed, thus giving additional stability.

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