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Thermodynamics of Denaturant-Induced Unfolding of a Protein That Exhibits Variable Two-State Denaturation †

✍ Scribed by Ferreon, Allan Chris M.; Bolen, D. W.


Book ID
121867812
Publisher
American Chemical Society
Year
2004
Tongue
English
Weight
260 KB
Volume
43
Category
Article
ISSN
0006-2960

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The urea and guanidine hydrochloride (GdnHCl)-induced denaturation of tetrameric concanavalin A (ConA) at pH 7.2 has been studied by using intrinsic fluorescence, 8-anilino-1-naphthalenesulfonate (ANS) binding, far-UV circular dichroism (CD), and size-exclusion chromatography. The equilibrium denatu