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Thermodynamic studies on interactions between DNA and dye

✍ Scribed by Akihiro Kagemoto; Akira Kunihiro; Yoshihiro Baba

Publisher: Elsevier Science
Year: 1994
Tongue: English
Weight: 633 KB
Volume: 242
Category: Article
ISSN: 0040-6031
DOI: 10.1016/0040-6031(94)85009-7

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The interaction of the fluorescent dye thiazole orange (TO) with nucleic acids is characterized. It is found that TO binds with highest affinity to double-stranded (ds) DNA [log(K) É 5.5 at 100 mM salt], about 5-10 times weaker to single-stranded polypurines, and further 10-1000 times weaker to sing

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