Thermodynamic studies and binding mechanisms of cell-penetrating peptides with lipids and glycosaminoglycans
✍ Scribed by André Ziegler
- Book ID
- 113418217
- Publisher
- Elsevier Science
- Year
- 2008
- Tongue
- English
- Weight
- 515 KB
- Volume
- 60
- Category
- Article
- ISSN
- 0169-409X
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## Abstract Cell‐penetrating peptides (CPPs) are cationic peptides that are able to induce cellular uptake and delivery of large and hydrophilic molecules, that otherwise do not cross the plasma membrane of eukaryotic cells. Despite their potential use for gene transfer and drug delivery, the mode
A simple and rapid homogeneous enzyme-based binding assay is described to study the degree of interaction between glycosaminoglycans and various macromolecules/peptides. The method is based on the homogeneous inhibition of a highly positively charged enzyme, acid deoxyribonuclease II (EC 3.1.22.1),