𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Thermal stabilization of tissues and the preservation of protein phosphorylation states for two-dimensional gel electrophoresis

✍ Scribed by Gary B. Smejkal; Chiara Rivas-Morello; Jae-Hyung Robert Chang; Emily Freeman; Alexander J. Trachtenberg; Alexander Lazarev; Alexander R. Ivanov; Winston P. Kuo

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
379 KB
Volume
32
Category
Article
ISSN
0173-0835

No coin nor oath required. For personal study only.

πŸ“œ SIMILAR VOLUMES

A modification for the improved analysis
A modification for the improved analysis of differential protein accumulation by two-dimensional polyacrylamide gel electrophoresis
✍ Abul K. M. Ekramoddoullah; Yingchun Tan πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 93 KB πŸ‘ 3 views

Phenolic compounds present in conifer tissues interfere with the analysis of conifer proteins by twodimensional (2-D) gel electrophoresis, causing gel streaking and distortion of protein patterns. A protocol was established by modifying the Hochstrasser method and using the Millipore 2 D Investigato

Two-dimensional phosphate-affinity gel e
Two-dimensional phosphate-affinity gel electrophoresis for the analysis of phosphoprotein isotypes
✍ Eiji Kinoshita; Emiko Kinoshita-Kikuta; Mamoru Matsubara; Yuri Aoki; Shiori Ohie πŸ“‚ Article πŸ“… 2009 πŸ› John Wiley and Sons 🌐 English βš– 335 KB πŸ‘ 1 views

## Abstract Herein, we describe three kinds of 2‐DE using phosphate‐affinity PAGE for the analysis of phosphoprotein isotypes. The first dimension is a urea‐PAGE, IEF/NEPHGE, or SDS‐PAGE, which are widely used. The second dimension is a phosphate‐affinity SDS‐PAGE using a phosphate‐binding tag mole