Thermal conformational transformations of tropocollagen. II. Viscometric and light-scattering studies

✍ Scribed by P. L. Privalov; I. N. Serdyuk; E. I. Tiktopulo

• Publisher: Wiley (John Wiley & Sons)
• Year: 1971
• Tongue: English
• Weight: 847 KB
• Volume: 10
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.360101004

✦ Synopsis

It WRS showii by visconietric nie:istiremetits that :I tropo(~olI:tgeii soliiiioii a i low shear gradieiiis mtiiifesth el:isiic. feiitiires which can he cwiiiierted oiily wit h the existence of :I labile spatial siriiciiire which develops iii iinne i i i the s o l i i t i o i i . To jiidge t)y the coticeiniratioii depeiidetice of the rnie of formatioii of this sirii(*itire, it does not represetit a moleciilar iiet formed by direct coiii:tct of ni:tcroniolec~iiles. \lost likely i his striivtiire is it vesiili of stabilization of rn:ict~c~niolec~iiles at R ceriaiti tiistailre from each other. The stitdy of light scatteriiig ti>-iropocollageii soliitiotis denioiistraied that it does not (*ort,espotid with the scaileriiig by rigid rod-shaped iiiiits. .4tionn;ilies i i i i h e character o f light scatteriiig :we pi.ol)xt)ly the resiilt. of iiitermoleciilar in1eifei~etic.e prodiired by R spatial siiperriioleciil:~~~ st riictiii.e :tiid, i t i t iirii, iiidiritie that this siri1c.t lire is to some erieiit regiilar. Iii ihe pt'eseiice of s:ilis the elastic. feat i i i ~ i i i the tropocollageti s;oliitioii mid :tilomalies i i i light scatieriiig dis;ippe:w i i i R iiarrow rmige of temperatiires itiimediately before the provess of deiiittiimtioii which mikes it possible to cotic*liide thai t he siiperniolecitlar regiilar striictiii.e is disriipted it, this tempwwtiire raiige.